Disney shares fell 1.8 percent to $40.28 in after-hours trading.
The operator of television networks ESPN and ABC, a movie studio and theme parks, posted fiscal first-quarter revenue of $10.8 billion, a 1 percent gain from a year earlier.
Analysts on average had expected revenue of $11.2 billion for the quarter.
Disney's studio division had a surprise hit with modestly budgeted film "The Muppets", but suffered from tough comparisons with home video sales for "Cars 2" versus the smash "Toy Story 3" a year earlier. The studio's revenue fell 16 percent in the quarter to $1.6 billion.
Revenue at media networks, the company's largest unit, gained 3 percent to $4.8 billion. Within that unit, broadcasting saw a 7 percent dip and margins fell, Gabelli & Co analyst Brett Harriss said. "That's the biggest negative that I saw," he said.
The theme parks unit, which like media networks have held up through a struggling economy, rose 10 percent. "Disney continues to exhibit pricing power, which shows the strength and competitive advantage of that business," Morningstar analyst Michael Corty said.
(Reporting By Lisa Richwine; Editing by Bernard Orr)